Design of a Duet Expression Vector to Characterize Ligand-receptor Interactions

Ligand-receptor Interactions and Drug Design.

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Design of Oligosaccharide Libraries to Characterize Heparan Sulfate – Protein Interactions

Ligand-receptor Interactions

The formation and dissociation of specific noncovalent interactions between a variety of macromolecules play a crucial role in the function of biological systems. During the last few years, three main lines of research led to a dramatic improvement of our understanding of these important phenomena. First, combination of genetic engineering and X ray cristallography made available a simultaneous...

Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions

We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak inte...

Analysis of Receptor-Ligand Interactions.